Table 1 in West Palaearctic species of Euura Newman, 1837 (Hymenoptera, Tenthredinidae)

Table 1. Amplicons, primers and multiplex PCR mixes. The listed genes were amplified in two (mixes 1–2) or three (mixes 1–3) different multiplex PCR reactions. In a few species, some of the introns are missing in PGD, TPI, or EF1a F2.
AmpliconPCR mixPrimers (forward + reverse)Concentration in the primer mix (pmol/µl)Amplicon length (bp)# introns 1COI1SymF5 +A2590210870PGD1PGD326F + PGD_hym_intRb3.5751–1400+1TPI1TPI35F + TPI706Rv24~720–8502EF1a F11EF1a47F + EF1a1282R512340EF1a F2 21EF1a47F + EF1a1282R~1600–2400+3ITS 31ITS-p5 + ITS-4unR11~600–1900+2NaK2NaK_263Fv2 + NaK_1918Rv2316540HSP90 h12HSP90_59F + HSP90_1843Rv25.517800HSP90 h22HSP90_59F + HSP90_1843Rv2~1900–3500+2POL22POL2_797F + POL2_2569R417710EF1a F2 52EF1a167F + EF1a1213R + EF1a1213Ra4~1300–2200+2TRRAP2 6TRRAP_833F + TRRAP_2620R51774–17860HSP90 h2 73HSP90b_83F + HSP90_1843Rv24~1900–3500+2POL23POL2_104Fv2 + POL2_2569R5~2500–2600 81TRRAP3TRRAP_833F + TRRAP_3784R52938–29500
1 Number of introns in the amplicon region.
2 The primers match both copies of the EF1a, but amplification of EF1a F2 usually does not work because it is much longer than F1 copy due to introns.
3 The plant specific ITS primers were included in the mix 1 only occasionally to identify the hosts of larvae. Amplicon length in angiosperms is usually around 650–750 bp.
4 The primers match both copies of the HSP90, but amplification of HSP90 h2 usually does not work because it is much longer than h1copy due to introns.
5 Two EF1a F2 specific (do not match the F1 copy at the 3’ end) reverse primers are included due to variability at the 3’ end.
6 This TRRAP amplicon was included in the mix 2 later in the study and mix 3 was then abandoned.
7 The forward primer is specific to h2 copy (does not match the h1 copy at the 3’ end).
8 The intron length varies little in most Nematinae, but in some Hoplocampa it can be much longer, resulting in an amplicon length of more than 3300 bp.