Single-cell transcriptome analysis of small cell neuroendocrine tumor of the endometrium reveals ISL1 as a potential biomarker for diagnosis and treatment

In this study, we performed single-cell RNA sequencing on a specimen from a patient with endometrial neuroendocrine carcinoma pathologically diagnosed. By analyzing the data, we found that the expression of Islet-1 was significantly up-regulated in tumor cells. Furthermore, we verified it in neuroendocrine tumor cell lines, and obtained cells with high expression of Islet-1 and cells with low expression of Islet-1 by flow sorting. Cell function experiments were performed in vitro to verify that the cell proliferation and cell migration abilities of the high expression Islet-1 group were significantly higher than those of the low expression group. In summary, Islet-1 protein in neuroendocrine tumors could act as a promising biomarker for the tumorigenesis and progression of neuroendocrine tumors. NOT PROVIDED; REQUESTED