Investigating pollination strategies in disturbed habitats: the case of the narrow-endemic toadflax Linaria tonzigii (Plantaginaceae) on mountain screes.

Raw data concernig the pollination biology and genetic identity of Linaria tonzigii. It includes: Seed number, seed weight and seed viability per breeding treatment; Genetic identity of several DNA regions of Linaria tonzigii, vulgaris and alpina. Abstract

Plant mating system may reflect an adaptation to a habitat type, with self-pollination
being potentially common in unstable and disturbed conditions. We investigated the reproductive ecology
of an Alpine, narrow-range toadflax, Linaria
tonzigii (Plantaginaceae), occurring in steep and dynamic mountain screes.
We explored self-compatibility and spontaneous autogamy, seed viability, daily
nectar production, pollinator behaviour and pollen transfer in wild populations,
using hand-pollination treatments, quantification of nectar volume, viability Tetrazolium
assay, active pollinator sampling and video recordings, and UV-bright dust for pollen
substitution. After ex novo sequencing of several genetic regions of L. tonzigii, we performed a multi-marker
phylogenetic analysis of 140 Linaria species
and tracked the occurrence of the self-compatibility trait. Our results showed
that this species is self-compatible, pollinated mostly via spontaneous
autogamy and pollinator-mediated geitonogamy, and self-pollinated seeds are as
viable as cross-pollinated ones. Selfing could be due to pollinator rarity
because, despite the studied species providing a high nectar volume, wild bees,
moths and small beetles infrequently visited its flowers in the sparsely
vegetated scree slopes. In addition, a preliminary survey showed that genetic
diversity is low in the study plant. Moreover, the
phylogeny shows that self-compatibility is scattered in the tree, suggesting the
adaptive nature of this reproductive system in the genus Linaria. This
study contributes to supporting theories that high prevalence of selfing is an
adaptation to environments unfavourable to cross-pollination, and particularly where
pollinators are rare such as in perturbed, poorly vegetated high-elevation habitats.
Please cite: Biella et al. - Investigating pollination strategies in disturbed habitats: the case of the narrow-endemic toadflax Linaria tonzigii (Plantaginaceae) on mountain screes. Plant Ecology (2021)

原始数据涉及林奈亚属植物林奈亚（Linaria tonzigii）的授粉生物学和遗传身份。数据包括：每项育种处理的种子数量、种子重量及种子存活率；林奈亚（Linaria tonzigii）、林奈亚（vulgaris）和林奈亚（alpina）的多个DNA区域的遗传身份。摘要

植物的交配系统可能反映了对其生境类型的适应，在不稳定和受干扰的条件下，自交可能普遍存在。本研究调查了分布于陡峭且动态的山坡上的高山狭域飞蓬（Linaria tonzigii，Plantaginaceae）的生殖生态学。我们探讨了自交亲和性、自然自交、种子存活率、每日花蜜产量、授粉者行为和花粉转移，通过人工授粉处理、花蜜体积量化、活性四唑盐测试、活跃授粉者采样、视频记录和紫外增亮粉尘进行花粉替代等方法进行研究。通过对L. tonzigii的多个遗传区域进行从头测序，我们对140种林奈亚属植物进行了多标记系统发育分析，并追踪了自交亲和性特征的分布。结果显示，该物种具有自交亲和性，主要通过自然自交和介导的地理异交进行授粉，自交种子与杂交种子具有同等存活率。自交可能由于授粉者稀少所致，尽管研究物种提供了较高的花蜜量，但在植被稀疏的山坡上，野生蜜蜂、蛾和小甲虫很少访问其花朵。此外，初步调查显示，研究植物中的遗传多样性较低。此外，系统发育分析表明，自交亲和性在树状图中分散分布，表明该生殖系统在林奈亚属中的适应性。本研究有助于支持高自交率是适应不利于杂交授粉环境的理论，尤其是在授粉者稀少的环境中，如受干扰、植被贫瘠的高海拔生境。请引用：Biella等.- 调查受干扰生境中的授粉策略：高山狭域飞蓬Linaria tonzigii（Plantaginaceae）在山坡上的案例。植物生态学（2021）