Amplicon-based analysis of enrichment of Caulobacteraceae from the environment

Environmental samples of twelve different sampling sites were collected. Six samples were taken from an oligotrophic pond located in the northern part of Weende, Goettingen, Germany. These environmental samples derived from frogs lettuce (Groenlandia densa) (PM), pond water (PW), surface water near pond algae (WSA), surface water near frog s lettuce (WSP), surface water of reed (WSR) and surface water close from Weende River entrance (WSW). Additionally, three samples were collected from the Weende River nearby the oligotrophic pond. Those samples are river water (RW) and (mixed are different sizes) river stones (RS and MRS). Further, two samples were gathered from a eutrophic pond at the North Campus of the Georg-August University Goettingen, which are surface water (POW) and surface water of stale eutrophic pond (PSW). In addition, samples from a puddle close by the eutrophic pond were collected as well (PUW). These samples were used as inoculum for a 0.001% (w/v) peptone-based enrichment with and without methanol. Bacterial community compositions of the resulting cultures were analyzed based on the 16S rRNA gene amplicon sequencing of region V3-V4.