Time course of the miR-203 effect during reprogramming from somatic cells to pluripotent cells

The balance between pluripotency and differentiation is critical during development and regeneration. miR-203 is a microRNA previously involved in differentiation of different tissues as well as in tumor suppression in multiple malignancies. We have shown that miR-203 is able to promote differentiation of embryonic stem (ES) and induced pluripotent stem (iPS) cells without decreasing pluripotency. We have observed that transient expression of miR-203 significantly improves the efficiency of ES/iPS cells in the generation of quimeras and tetraploid complementation assays, in addition to inducing complex embryo-like structures when these pluripotent cells are injected in mice. In the present RNA seq, we intend to analyze the trascriptomic profile of miR-203 WT, KO and KI MEFs reprogrammed to inducible pluripotent cells (iPSCs) at different time points. Overall design: The general idea was to analyze the transcriptomic profile of miR-203 WT, KO and KI MEFs during reprogramming to iPSCs. We collected samples at different time points: MEFs, day 0 after inducing reprogramming, day 4, day 6 and day 7 of reprogramming and finally, iPSCs generated from miR-203 WT and KO MEFs.