Human endometrial cancer cell gene expression profiles of the estrogen receptor α (ERα) hinge region mutants (H1 and H2NES). Homo sapiens

ERa negative Ishikawa cells were stably infected with Era: Wild type ERa, H1 ERa, H2NES ERa the design was to determine if rapid action non-genomic mediated signals would lead to gene expression changs. Overall design: Four stable cell lines, vector, WT, H1 and H2NES ERa were treated for 4hr or 24 hr with 10nM Estradiol or vehicle, n=3 for each treatment group