eNOS associates with Caveolin-1

Caveolin-1 is the primary negative regulatory protein for eNOS. Caveolin-1 binding to eNOS compromises its ability to bind Calmodulin (CaM), thereby inhibiting enzyme activity. The major binding region of caveolin-1 for eNOS is within amino acids 60-101 and to a lesser extent, amino acids 135-178 (Garcia-Cardena et al. 1997; Ghosh et al. 1998).

Caveolin-1 蛋白作为 eNOS 的主要负向调控因子，其与 eNOS 的结合会损害其与钙调蛋白（CaM）的结合能力，从而抑制酶的活性。Caveolin-1 与 eNOS 的主要结合区域位于氨基酸序列的 60-101 位，次之为 135-178 位（Garcia-Cardena 等，1997；Ghosh 等，1998）。