Knockdown and sequencing of Nogo-B in endothelial cells

Endothelial cells are the critical targets of injury in Diabetic nephropathy (DN) and endothelial cells lesions contribute to the disease progression. Neurite outgrowth inhibitor B (Nogo-B) as an endoplasmic reticulum (ER)-resident protein, could mediate ER stress, playing a pivotal role in vascular remodeling after injury and maintaining the structure and function of the endoplasmic reticulum. Yet Nogo-B regulation of ER stress and endothelial cells injury remains largely unknown. Related studies have shown that changing the level of ER stress in glomerular endothelial cells could affect autophagy. Herein, we investigated whether ER stress-mediated autophagy causes endothelial dysfunction in the diabetes.