Methamphetamine Exposure Drives Cell Cycle Exit and Aberrant Differentiation in Rat Hippocampal-derived Neurospheres

In the present study, we applied three-dimensional (3D) neurospheres derived from the embryonic rat hippocampal tissue to investigate the effect of methamphetamine on neurodevelopment. Neurospheres were randomly divided into three groups: control, 1.5 mM METH group and 3 mM METH group, which were treated with solvent, 1.5 mM methamphetamine and 3 mM methamphetamine for 24h, respectively. Then neurospheres were collected for whole genome transcriptional analysis after METH treatment.