Genomic instability of Jurkat clone E6-1 T-cell lines affect gene expression and function

Jurkat clone E6-1 is a human T-cell line derived from a paediatric case of acute leukaemia, and is frequently employed as a model T-cell for immunology research. In this study, we characterised genomic, transcriptomic, and functional features of Jurkat clone E6-1 cells stored in three different laboratories, and compared these to Jurkat E6-1 cells from the ATCC. Cell aliquots were cultured in identical conditions, and were analysed using cytogenomic approaches, whole exome and whole transcriptome sequencing, flow cytometry, and multiplex cytokine bead array.