MicroRNA Expression in Kidney Development Comparing E14.5 and P1

This study was performed to identify miRNAs that are differentially regulated during mouse kidney development. Two stages (E14.5 and P1) were analyzed. Differential expression was verified using qPCR and/or in situ hybridization. Multiple kidneys were dissected, pooled and processed for total mRNA isolation. Probe labeling, hybridization and data analysis were performed by Exiqon. The three submitted sample files are technical replicas using the same mRNAs.本研究旨在筛选在小鼠肾脏发育进程中呈现差异调控的微小RNA（microRNAs，miRNAs）。本次分析涵盖两个发育阶段：胚胎期14.5天（E14.5）与出生后第1天（P1）。研究通过实时定量聚合酶链反应（quantitative PCR，qPCR）和/或原位杂交（in situ hybridization）对差异表达情况进行了验证。研究人员对多份肾脏组织进行解剖、混合后，开展总mRNA的提取工作。探针标记、杂交及数据分析工作均由Exiqon公司完成。本次提交的三份样本文件均为使用同一批mRNA制备的技术重复样本。