Psylloidea phylogenomics

Resolving the psyllid tree of life and understanding evolutionary relationships in the superfamily Psylloidea is challenging due to the lack of clear morphological synapomorphies for many groups. Shot-gun sequencing using mixed pool DNAs for more than 400 species resulted in recovery from de novo assemblies of near complete mitogenomes (≥ 10 kb) for 359 species, and partial genomes (5-10 kb) for an additional 40 species. The resulting phylogeny improves and clarifies the family classification and resolves some of the long standing uncertainties in relationships within and between genera. A whole nuclear genome scan approach using the Anchored Hybrid Enrichment (AHE) method (total 447 loci with average length 760 bases from an estimated 373 nuclear genes) for a representative subset of taxa confirms the placement of major groupings and overall tree topology found with the mitochondrial data. The data generated represent a major increase in molecular resources for this superfamily.