Genome-wide survey by ChIP-seq reveals CREB1 regulation of lncRNAs in gastric cancer.

cAMP responsive element binding protein 1 (CREB1) is a transcription factor belonging to the basic leucine zipper (bZIP) family. Previous studies on the transcriptional regulatory targets of CREB1 have focused on protein-coding genes. The non-coding targets regulated by CREB1 in gastric cancer remain to be further studied. Herein, the genome-wide mapping of CREB1 binding in gastric cancer cell AGS was determined by chromatin immunoprecipitation sequencing. Overall design: AGS cell lysates were sonicated and immunoprecipitated with anti-CREB1 antibody (Cell Signaling, MA, USA, Cat# 9197, rabbit monoclonal). ChIP-sequencing analysis was performed on two samples: AGS-IP and AGS-Input.