Scripts and datasets to generate or analyze data

Chronic venous thrombosis is difficult to treat with current approaches, in part due to its extensive collagen network. Histotripsy is a focused ultrasound therapy that uses bubbles to disrupt tissue, and has several features that make its use for chronic thrombosis attractive. This study used a porcine model to test the hypothesis that portions of chronic thrombosis undergoing active remodeling can be disrupted by histotripsy. Vessels occluded for 30 days were found to be composed primarily of disorganized collagen, and cells that promoted disorganized collagen structures. Histotripsy pressure pulses were able to generate bubbles in all cases, resulting in a 59% mean reduction in the thrombus area. Further, disorganized collagen and cells within the samples were damaged to a greater extent than organized collagen. These data indicate there are chronic venous thrombosis components susceptible to mechanical perturbation by histotripsy despite the extensive collagen.<br>These files were used for analysis of data.