Sanger sequencing files of SCNN1A exon 2-3-4 of Afro-Colombian patients with apparent resistant hypertension
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We studied the prevalence of genetic variants in the ENaC genes and their association with renin-aldosterone profiles in Afro-Colombian patients with apparent resistant hypertension. From our study population we selected 7 random samples for the Nanopore sequencing of most of the coding region of the SCNN1A gene. As all the participants were from the same Colombian town of Puerto Tejada, we wanted to rule out the possibility of genetic variants common to this specific population. However, amplifying through conventional PCR the exon 2-3-4 SCNN1A region in one amplicon was difficult as the PCR product was full of unspecific amplicons. To solve this problem, we decided to perform sanger sequencing individually in these exons in those 7 samples. These are the ab1 files of the electropherograms.



