File S1 - MHC-I Molecules Selectively Inhibit Cell-Mediated Cytotoxicity Triggered by ITAM-Coupled Activating Receptors and 2B4

Supporting figures. Figure S1, Crosslinking the NKL cell surface receptors CD58, CD54 (ICAM-1), CD50 (ICAM-3), CD29, CD44, CD2 and CD25 with the killer activating receptors, CD16, NKG2D and NKp46 did not significantly decrease the NKL cell-mediated cytotoxicity against P815 cells. Figure S2, MHC-I engagement augments NKL/P815 cell conjugation. Exponentially growing Ca-AM-stained (calcein acetoxymethylester) NKL cells were co-cultured with HE-stained (hydroethidine) P815 cells plus mAb against Killer Activating Receptors or Inhibitory receptors at 1∶2 E/T ratio. (DOCX)