Allelic specific expression (ASE), allelic imbalance (AI) determination, and false positive rate for blastomeres in 4-cell and 8-cell embryos.

The locus counts of ASE and AI in individual cells of early embryos. Loci were selected based on the following criteria: (1) Sequence data produced multiple nucleotides; (2) Observed alleles are listed in dbSNP; (3) The position is covered by at least 25 uniquely aligned reads; (4) No more than 50% of covering reads supporting each nucleotide align to identical positions in the genome; (5) UCSC (University of California Santa Cruz) annotation supports transcription of only one strand.