RNA seq of liver homogenates collected every 4 hours for one 24 hour cycle from adipocyte knockouts for Bmal1

We used RNA seq to evaluate gene expression oscillations in liver from mice lacking Bmal1 in adipocytes. We collected liver from adipocyte specific Bmal1 knockout mice every 4 hours throughout one 24 hour cycle. Mice were housed at room temperature, 12 hour light, 12 hour dark schedule and were fed ad libitum before the experiment. Mice were kept at constant darkness and were fasted for 24 hours prior tissue collection本研究采用RNA测序（RNA-seq）技术，对脂肪细胞缺失Bmal1的小鼠肝脏组织的基因表达振荡情况进行评估。我们在完整的24小时周期内，每4小时采集一次脂肪细胞特异性Bmal1敲除小鼠的肝脏样本。实验前，小鼠饲养于室温环境，遵循12小时光照、12小时黑暗的光周期制度，并可自由采食；在组织采集前，小鼠被置于持续黑暗环境中并禁食24小时。