CRISPR/Cas9 technology has revolutionized genetic engineering of primary cells. Although its use is gaining momentum in studies on CD8+ T cell biology, it remains elusive to what extent CRISPR/Cas9 af

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File S2.zip

CRISPRi基因编辑

基因敲入序列分析

Annotated sequence files in .ape format (comparable with Genbank) of knock-ins generated by CRISPR/Cas9-mediated genome editing. Unmodified genomic sequence is also provided.

Figshare2023-03-14 更新20

Guide RNA engineering enables efficient CRISPR editing with a miniature Syntrophomonas palmitatica Cas12f1 nuclease

CRISPRi基因编辑

GuideRNA工程

To confirm the tolerance of SpaCas12f1, mismatched assay was performed for NGS.

NIAID Data Ecosystem50

CRISPR RNA-seq from HepG2 (ENCSR194SPW)

CRISPRi基因编辑

肝癌基因表达

Control CRISPR gRNA against no target in HepG2 cells followed by RNA-seq. (NT-BGHcLV07-B) For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov

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Homo sapiens Raw sequence reads

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