Effects of Hnf4a deficiency on H3K4me2, H3K4me3, H3K9me2, H3K9me3, H3K27me3 and H3K4ac at the specific loci in female mouse livers determined by the improved ChIP-qPCR.

a The enrichment of histone modification at the specific locus (relative to input) in livers of Hnf4a-LivKO is divided by that in wild-type to calculate the fold of alteration. The number signifies the fold of alteration that is significant in Hnf4a-LivKO mice (p Hnf4a-LivKO mice. Fig. S1, Fig. S2 and Fig. S3 inFile S1 show the same data in the column plots.b The genes harboring loci are categorized into same groups as those in Fig. 4: Up-regulated (↑), down-regulated (↓) and unchanged (–), which are separated by dotted lines.c Additional loci for H3K4me3 were tested, including Cyp2c44 exon1, Cyp2c44 exon2, Sult1b1 exon1, Sult1b1 exon2, Sult1e1 exon2, Ugt2b1 exon1 and Ugt2b1 exon2. Among these loci, the alterations at Cyp2c44 exon1, Ugt2b1 exon1 and Ugt2b1 exon2 are significant, with the fold of 0.2, 0.5 and 0.3, respectively.