Quality of fragment assembly with two levels of coverage.

Read length = 200 bp, Total error rate = 1.0%We assembled the whole genome of D. melanogaster and human chromosomes 1, 11, and 21 at two different levels of coverage of 11.25x and 20.0x. We report the proportion of sequence covered by our assembly, the reasons for missing sequence coverage, the contig N50 length statistic, the base quality, and the rates of misassembly incidents and small insertions and deletions. Contig N50 lengths were large even at 11.25x coverage, growing to approximately triple the size for 20.0x coverage. The misassembly rate was comparable to those found in other draft assemblies, while small indels were due to tandem repeats.