Combined transcriptome and T cell receptor alpha/beta (TRA/TRB) single cell RNA sequencing of donor-reactive T cells in stable kidney transplant recipients before and 3-5 years post-transplant.

A combined single cell RNA sequencing approach determined both transcriptomic profiles and complete V(D)J variable regions of TR transcripts from individual alloreactive T cells of stable kidney transplant recipients (N=7) prior to and 3-5 years after transplantation Peripheral blood mononuclear cells (PBMCs) were stimulated with CD3-negative PBMCs of their donor or a third party control. Following stimulation, alloreactive T cells were isolated from PBMCs by Fluorescence-activated cell sorting (FACS) according to the presence CD137 signal and analyzed using scRNAseq.