Comparison between chromatin purification methods.
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(a.) Chromatin was purified from HeLa cells through three different preparations. (b.) SDS-PAGE analysis of a crude nuclear lysate and the chromatin preparations. Approximately 10 ug of protein were loaded in each lane. Supernatant and pellet fractions are shown separately for the salt extraction and the MNase digestion protocols. (c.) Venn Diagram indicating the number of proteins detected in one, two or all three chromatin preparations. Where applicable, preparation fractions (supernatant and pellet) were combined.
创建时间:
2011-09-14



