Fasciola hepatica miRNAs in mouse peritoneal Ago2

Helminths, or worms, are multicellular parasites that can live for many years within their vertebrate hosts. Of prime importance is the regulation of the host immune cell signalling pathways to prevent the parasite's elimination before they can produce their off-spring in the form of eggs. Fasciola hepatica, a global worm parasite of humans and their livestock, regulates host innate immune responses within hours of infection. Host macrophages, essential to the first-line defence mechanisms, are quickly restricted in their ability to initiate a classic protective pro-inflammatory immune response. To determine the role of both host miRNAs and parasite-derived miRNAs in this outcome to infection with F. hepatica, peritoneal macrophages were harvested from the peritoneal cavity of BALB/c mice at various timepoints during infection and subject to RNASeq. Sequenced Ago2 extracted from peritoneal macrophages of fasciola infected mice Overall design: RNA extracted from infected mouse peritoneal macrophages sequenced after library prepartion for miRNAs