GmFT4 expression is associated with flowering time, E1 genotype and E1 expression.

Plants were grown in a climate chamber under either SDs (12 h:12 h light/dark) or LDs (16∶8 h light/dark). Fully expanded trifoliolate leaves were sampled 4 h after beginning of light phase from three individual plants. Relative expression level of GmFT4 and E1 were analyzed by real-time RT-PCR. Transcript levels relative to TUA5 are represented in each treatment, s.d. represents standard deviation. Soybean cultivar Kariyutaka under SDs was used as control whose expression level was set to 1 for all genes analyzed. Values represent means of three biological replicates. Genotype E1 is considered as functional WT allele, the e1-as allele represents a partially functional allele and the e1-fs alleles are nonfunctional allele.