Studies on bleomycin-induced lung fibrosis in mice. Studies on bleomycin-induced lung fibrosis in mice

We perfomed a microRNA microarray on to assess differentially expressed miRNAs in bleomycin-induced lung fibrosis in mice. To induce pulmonary fibrosis, belomycine (Sigma-Aldrich, St Louis, MO) was dissolved and administred intratracheally at a dose of 1.5U/kg body weight. Control animals received saline only. 21 days post bleomycin treatment, mice were sacrificed and lung tissue were collected for RNA extraction and microRNA microarray. Overall design: C57Bl6 mice were treated with either 1.5U/kg bw bleomycin (N=3) or saline (n=3) and after 21 days, lung tissue were dissected out and RNAs were extracted and miRNA array was performed.