Single-cell multiomic analysis revealed the transcriptional and epigenetic regulatory characteristics of esophageal squamous cell carcinoma

We performed scATAC on tumor tissues and adjacent tissues of four groups of ESCC patients with different clinical stages, drew the full-cycle chromatin accessibility map of ESCC, studied the effect of epigenetic changes in chromatin accessibility on ESCC heterogeneity, revealed the changes in chromatin accessibility during the evolution of tumor-associated fibroblasts and tumor-associated macrophages in ESCC TME, and revealed the epigenetic mechanism of CD8+ depleted T cells involved in tumor immunosuppression.