The MIP (MIPFOC01) was mixed with different amounts of Foc genomic DNA (160 ng, 80 ng, 40 ng, 20 ng, 10 ng, 5 ng, 2.5 ng and 1.25 ng) in triplicates and analyzed in a MIP assay. The PCR amplicon was e
Whole-genome sequencing of Mafiga virus, Ngerengere virus, and Songea and Berega virus detected in Lemniscomys rosalia, Mus minutoides and Grammomys surdaster, respectively.
1LOD, limit of detection; LOQ, limit of quantification; values are calculated assuming maximal injection volume;2The UV signal for was correlated with fluoresence for enhanced sensitivity.