Microarray dataset of gene transcription in mouse microglia and peripheral monocytes in contextual fear conditioning

Using Illumina MouseWG-6v2 microarrays, we investigated the gene transcription changes in microglia and peripheral monocytes after contextual fear conditioning of C57BL/6J mice. Mice were trained with or without a single minimized footshock stimulation (0-s or 2-s, 0.4 mA) and re-exposed to the training context without footshock for three different durations 24 h later: 0 min (FS0), 3 min (FS3), or 30 min (FS30). Whole brain microglia and peripheral monocytes were prepared 24 h after re-exposure using a neural tissue dissociation kit, including non-footshock controls for two re-exposure durations (Con3 and Con30). The data can be valuable for researchers interested in glial cells and neurotransmission studies and are related to the research article "Contextual fear conditioning regulates synapse-related gene transcription in mouse microglia"本研究采用Illumina MouseWG-6v2微阵列（Illumina MouseWG-6v2 microarray），探究C57BL/6J小鼠经情境恐惧条件反射（contextual fear conditioning）后，其小胶质细胞（microglia）与外周单核细胞（peripheral monocytes）内的基因转录变化。小鼠接受单次最小化足底电击刺激（时长0秒或2秒，电流0.4mA）训练或不接受刺激训练，并于训练后24小时重新暴露于训练环境且不施加电击，暴露时长分为三个实验组：0分钟（FS0）、3分钟（FS3）与30分钟（FS30）。在重新暴露24小时后，本研究采用神经组织解离试剂盒（neural tissue dissociation kit）分离全脑小胶质细胞与外周单核细胞，同时设置两种暴露时长的非足底电击对照组（Con3与Con30）。本数据集可供关注胶质细胞与神经传递研究的科研人员参考，其关联研究论文为《情境恐惧条件反射调控小鼠小胶质细胞中突触相关基因转录》（Contextual fear conditioning regulates synapse-related gene transcription in mouse microglia）