In cell LIGR-seq. Influenza A virus
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https://www.ncbi.nlm.nih.gov/bioproject/PRJDB12485
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We performed LIGR-seq in cells infected with the influenza virus. To concentrate viral RNAs in cells, we performed pull-down of viral RNAs by biotinylated antisense ssDNA. 87mut virus has mutations in segment 5 to disrupt the secondary RNA structure of the region. 87rec virus has additional mutations in segment 5 to reconstitute the secondary structure. We set ligation(-) control to negative control samples.
创建时间:
2023-10-19



