Integrative genome-wide analysis reveals the RNA-binding motifs of yeast Pcf11 and its role in fine-tuning terminator strength

Pcf11 is a transcriptional termination factor that interferes with cell survival and mRNA 3′-end processing. However, the mechanism by which Pcf11 regulates transcriptional termination and the molecular basis of its interaction with the terminator remain unclear. Here, we performed gene expression profile, binding pattern, and RNA-binding motif studies on Pcf11. We used in vivo CLIP-seq to map thousands of Pcf11 binding sites in Saccharomyces cerevisiae and revealed the RNA-binding motifs of Pcf11 in the 3'-UTR region.Pcf11是一种转录终止因子，可干扰细胞存活及mRNA 3'端加工。然而，Pcf11调控转录终止的具体机制，以及其与终止子相互作用的分子基础仍未阐明。本研究针对Pcf11开展了基因表达谱、结合模式以及RNA结合基序相关研究。我们采用体内交联免疫沉淀测序（in vivo CLIP-seq），在酿酒酵母（Saccharomyces cerevisiae）中定位了数千个Pcf11结合位点，并揭示了Pcf11在3'非翻译区（3'-UTR）中的RNA结合基序。