Circadian Reprogramming by High-Fat Diet Signifies Temporal Coordination of Metabolic Cycles in the Kidney

The circadian gene expression in peripheral tissue displays rhythmicity which is reprogrammed by feeding rhythms and dietary composition in mammals. In this study, circadian transcriptome was performed to investigate how high fat diet influences circadian gene regulation in the mouse kidney. 6-week-old, male C57BL/6 mice were subjected to ad libitum normal chow diet or to high-fat diet under 12hr light/ 12hr dark schedule. Kidney was harvested every 4 hours over the circadian cycle at ZT0, 4, 8, 12, 16, 20 (n=3 per time point per group). Total RNA was extracted from kidney, and used for RNA-seq.哺乳动物外周组织的昼夜节律基因表达（circadian gene expression）呈现节律性特征，且该节律可被进食节律与膳食组成重塑。本研究开展昼夜节律转录组测序（circadian transcriptome），以探究高脂饮食（high-fat diet）对小鼠肾脏昼夜节律基因调控的影响。选取6周龄雄性C57BL/6小鼠，在12小时光照/12小时黑暗的光周期条件下，分别给予自由采食的普通维持饲料或高脂饮食。在昼夜节律周期内，每隔4小时于授时因子时间（Zeitgeber Time, ZT）0、4、8、12、16、20处采集肾脏组织，每组每个时间点设置3个生物学重复（n=3）。从采集的肾脏组织中提取总RNA，用于RNA测序（RNA-seq）。