Sequence Data

To verify some of the significant CpGs from the genome-wide analysis, we selected the target CpGs at the warfarin-associated genes. Thereafter, we used pyrosequencing to detect the methylation levels of CpGs with more samples in the validation cohort (low-dose group: 37; medium-dose group: 59; and high-dose group: 35). DNA was extracted from peripheral blood cells using the TIANamp Blood DNA Kit (Cat. #DP318, Tiangen, China) and DNA bisulfite conversion was conducted in a bisulfite batch with the EpiTect Bisulfite Kit (Cat. #59104, Qiagen, German); then, the converted DNA used as the template for PCR amplification. All primers for PCR and sequencing were designed by PyroMark Assay Design 2.0 Software (version 2.0.01.15; Qiagen, German). Pyrosequencing was carried out by PyroMark Q24 (Qiagen, German). The EpiTect Control DNA and Control DNA Set(Cat. #59695, QIAGEN; German) was used in the pyrosequencing. In addition,the PyroMark Q24 Validation Oligo(Cat. #979203, QIAGEN; German) was also used to check the performance of the PyroMark Q24 system.