Longitudinal DNA methylation and RNA-seq datasets in primary human fibroblasts with mitochondrial genetic, metabolic, and endocrine perturbations. Longitudinal DNA methylation and RNA-seq datasets in primary human fibroblasts with mitochondrial genetic, metabolic, and endocrine perturbations

The current RNA sequencing and DNA methylation datasets provide an epigenomic map across the entire replicative lifespan of primary human fibroblast cell lines from n=6 healthy male and female donors and n=3 donors with lifespan-altering mitochondrial disease caused by mutations in the SURF1 gene. These datasets can be integrated with other measures collected in parallel along the lifespan, including cytological (cell size, morphology), bioenergetic (energy expenditure, derived ATP synthesis rates from Seahorse), secreted proteins, telomere length, and whole-genome sequencing (WGS) data. This dataset also includes experimental manipulations with treatments targeting oxidative phosphorylation (OXPHOS) and glycolysis, and glucocorticoid signaling, providing an opportunity to examine the influence of stress and bioenergetics on human aging biology. All processed data can be accessed and browsed at our webtool: https://columbia-picard.shinyapps.io/shinyapp-Lifespan_Study/ Overall design: Refer to individual Series