Murine Fgf3-/-;Fgf10-/- vs. Fgf3+/-;Fgf10+/- otic placode profiling

Transcriptional profiling of mouse 4-8 somite microdissected otic placodes from Fgf3/Fgf10 double heterozygotes (controls) compared to Fgf3/Fgf10 double null mutants. The purpose was to determine the effectors that are induced (or repressed) by Fgf3 and Fgf10 in the course of otic placode induction. Two condition experiment, double heterozygote vs. double null placodes. Biological replicas: 3 Fgf3+/-;Fgf10+/- and 3 Fgf3-/-;Fgf10-/- pools of ten placodes each.本数据集针对小鼠4-8体节显微解剖获取的耳基板开展转录组谱分析，样本分别取自Fgf3/Fgf10双杂合子（对照组）与Fgf3/Fgf10双纯合缺失突变体，并对两组样本进行对比分析。本研究的核心目的是鉴定在耳基板诱导过程中，受Fgf3与Fgf10诱导（或抑制）的效应分子。本实验采用双组对照设计，对比组为双杂合子耳基板与双纯合缺失突变体耳基板。生物学重复设置如下：每组各设置3个混合样本，每个混合样本包含10个耳基板，其中对照组样本基因型为Fgf3+/-;Fgf10+/-，突变组样本基因型为Fgf3-/-;Fgf10-/-。