Expression of Brn3a, Islet-1 and βIII-tubulin in radial retinal sections and co-localisation of staining for the astrocyte marker GFAP, the amacrine cell marker Syntaxin-1 and the macrophage/microglia marker ED1.

Immunohistochemically stained 20-µm-thick radial sections of retina, stained for Brn3a (green; Ai, Aiv and Avii), Islet-1 (green; Aii, Av and Aviii), βIII-tubulin (green; Aiii, Avi and Aix), GFAP (red; Ai, Aii and Aiii), Syntaxin-1 (red; Aiv, Av and Avi) and ED1 (red; Avii, Aviii and Aix), taken from Group 2a eyes (intact optic nerve), with the outer nuclear layer (ONL), inner nuclear layer (INL) and ganglion cell layer (GCL) labelled, insert showing higher power image of the GCL. All images are representative; DAPI (blue) was used as a nucleus stain (scale bar: 50 µm; inset scale bar: 25 µm).