Tissue resident and bone marrow derived macrophages in murine lung tumors. Tissue resident and bone marrow derived macrophages in murine lung tumors

We isolated macrophages from murine lung tumors and their in vitro derived progenitors for RNA sequencing Overall design: Lewis lung carcinoma tumors grown in mice were used to isolate tissue resident CD45+CD11b+Gr1-F4/80hi and bone marrow derived CD45+CD11b+Gr1+F4/80lo macrophages for RNA sequencing. These cells were also used to generate macrophage progenitors by culture in methycellulose + stem cell growth factors. Resulting Colony cells were then sorted for Kit expression and sequenced.