Expression of receptors for inflammatory mediators in murine vagal nodose versus jugular capsaicin-sensitive (Trpv1-positive) C-fiber neurons

Capsaicin-sensitive (Trpv1-positive) sensory C-fibers derived from vagal ganglia innervate the visceral organs, and respond to inflammatory mediators and noxious stimuli. These neurons play an important role in maintenance of visceral homeostasis, and contribute to the symptoms of visceral inflammatory diseases. Vagal sensory neurons are located in two ganglia, the jugular ganglia (derived from the neural crest), and the nodose ganglia (from the epibranchial placodes). The functional difference, especially in response to immune mediators, between jugular and nodose neurons is not fully understood. In this study, we microscopically isolated murine nodose and jugular capsaicin-sensitive / Trpv1-expressing C-fiber neurons and performed transcriptome profiling using ultra-low input RNA sequencing. Examination of gene expression in nodose and jugular capsaicin-sensitive C-fiber neurons源自迷走神经节的辣椒素敏感（TRPV1阳性）感觉C纤维可支配内脏器官，并可对炎症介质与伤害性刺激产生应答。这类神经元在维持内脏稳态中发挥重要作用，同时参与内脏炎症性疾病的症状进程。迷走感觉神经元存在于两处神经节：颈静脉神经节（源自神经嵴）与结状神经节（起源于鳃上基板）。目前，颈静脉与结状神经元之间的功能差异，尤其是在对免疫介质的应答方面，尚未完全明确。本研究通过显微镜分离得到小鼠结状及颈静脉来源的辣椒素敏感/表达TRPV1的C纤维神经元，并采用超低起始量RNA测序技术开展转录组谱分析。对结状及颈静脉辣椒素敏感C纤维神经元的基因表达进行了分析