Knockdown analysis of <i>CPIJ005623</i> in <i>C. molestus</i> Italy females.

A. Diagram representing experimental design of knockdown (KD) experiments, d-day post pupal eclosion, T-time point. B. KD assessment of iCPIJ005623 in Italy ovaries. Reduction of CPIJ005623 mRNA levels similar to uninfected ovaries was observed in infected ovaries when CI developmental progression was seen. C. Picture examples of developmental progression in Culex embryos undergoing CI, Stage I: early arrest, Stage II and III: late arrest. D. Increased percentage of unhatched embryos mimic a reduced rescue function. Also increased early embryo (stageI) developmental arrest is detected after iCPIJ005623 in compatible Italy male cross.