ADF/cofilin promotes invadopodial membrane recycling during cell invasion in vivo

For Fluorescence Loss in Photobleaching (FLIP), selected ROIs (~1μm) of the anchor cell (AC) in worms expressing cdh-3 > LMP-1::GFP were bleached every 30 seconds for 10 minutes at 100% power for 20 iterations, and images were taken prior to and after each beaching interval. For Fluorescence Loss in Photobleaching (FLIP), selected ROIs (~1μm) of the anchor cell (AC) in worms expressing cdh-3 > LMP-1::GFP were bleached every 30 seconds for 10 minutes at 100% power for 20 iterations, and images were taken prior to and after each beaching interval. For the unc-60a (RNAi) animal shown here, the LMP-1::GFP signal (white) persisted approximately 10 minutes after initiation of photobleaching.