Proteomes of acrosomes and acrosome-reacted sperm of the house mouse

Using label-free nano-scale liquid chromatography tandem mass spectrometry (nLC-MS2) proteomics, we provide proteomes of the acrosomes and acrosome-reacted sperm isolated from cauda epididymis of free-living house mice Mus musculus musculus. Among all proteins, 334 proteins were significantly enriched in the acrosome thus representing 27.3% of the whole proteome of the intact sperm. Importantly, we have detected a total of nine calycins while eight of them belong to the lipocalin protein family. Column names in resulting tables are tagged as S1-S10 (supernatants) and represent acrosomal content (A1-A10 in Table S1) while samples P1-P10 are pellets and represent arcosome reacted sperm (S1-S10 in Table S1).