Both the host microbiome and the microbiome of the built environment can have profound impacts on human health. While prior studies have suggested that the variability introduced by DNA extraction met
In this study, we examine several commonly used protocols for extracting bacterial DNA from ants to assess the biases inherent in each method. Bacterial community composition recovered using Illumina
Epidemiologic studies use various biosample collection methods to study associations between human oral microbiota and health outcomes. However, the agreement between the different methods is unclear.
we evaluated the bias of nested PCR in estimating microbial diversity and community structure by comparing it with standard PCR. We investigated the influence of microbial diversity (relatively low vs