Shotgun sequencing of ten archaeological individuals from the archaeological site of Histria, Romania

In this study, we employed shotgun sequencing techniques to delve into the genetics of ten archaeological individuals who were interred in the Roman necropolis of Histria. Histria was a city established by Greek colonists and is located near the Black Sea and the mouth of the Danube. Our project explores various facets of their genetic makeup. First, we performed ancient DNA authentication was rigorously conducted to ensure the reliability of the genetic data. We also estimated the sex of these individuals using genomic technique, recognizing the nuances of skeletal preservation and the limitations of traditional methods. Moreover, contamination estimates were meticulously assessed to distinguish endogenous DNA from potential exogenous sources. Finally, we investigated the intricate details of population structure of this sample. This multi-faceted approach offers a holistic view of the genetic landscape of this unique archaeological cohort. Because we upload the raw fastq files, we urge researchers that use our data to perform their own filtering and quality assessments before including the data into their downstream analyses.