Transcriptional profiling of mouse cavenous endothelial cells in normal and high glucose condition. Transcriptional profiling of mouse cavenous endothelial cells in normal and high glucose condition

Purpose: To investigate the potential target genes associated with diabetic condition in mouse cavernous endothelial cells (MCECs) for diabetic induced erectile dysfunction (ED) treatment. Materials and Methods: Mouse cavernous tissue was embedded into Matrigel and sprouted cells were subcultivated for other studies. To mimic diabetic condition, the MCECs were exposed to normal-glucose (NG, 5 mmoL) and high-glucose (HG, 30 mmoL) condition for 72 hours. RNA-sequencing assay was performed to evaluate gene expression profiling and RT-PCR was used for sequencing data validation. Overall design: normal glucose ( n=2) and high glucose ( n=2)