Differential gene expression in IDH1-R132H Tumor Neurospheres generated from a mouse model of Low Grade Glioma. Mus musculus

IDH1-R132H is expressed in Low Grade Glioma (LGG) in combination with mutation in ATRX and TP53 genes. IDH1-R132H results in gain of function with production of 2-hydroxygluatrate, that in turn generates a hypermethylatyed phenotype in DNA and histone with consequences in epigenetic regulation of gene expression. Here we will compare the gene expression profile between IDH1-R132H and IDH1 Wt tumor neurospheres. Overall design: Evaluate differential gene expression between tumor neurospheres IDH1-R132H and IDH1 wt.