RNAseq of iPSC clones derived from patient CD34+ cells from peripheral blood in the XSCID gene therapy clinical trial (NCT01306019). Homo sapiens

RNAseq of iPSC clones derived from patient CD34+ cells from peripheral blood in the XSCID gene therapy clinical trial (NCT01306019). These iPSC clones harbor various lentivector integration sites in different genes and the RNAseq analysis was carried out to identify fusion transcripts of host genes and the integrated vector in each clone. Other lineage specific cells were also isolated from patient peripheral blood. The iPSC clones were single-cell derived clones and all cells in each clone carry same vector integrations. The lineage specific cells isolated from peripheral blood were polyclonal for vector integrations.