GRN knockout, sorted brain cells
收藏干细胞与再生医学数据中心2022-02-20 更新2024-03-06 收录
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1 year old mice were perfused and brains were dissociated. Cells were fixed, immunolabeled and FACS sorted. RNA was extracted from neuron, astrocyte, and microglial cell populations. Typical RIN=4-5 for neurons, 6-8 for astrocytes, and 5-7 for microglia. Typical RNA yields ~100ng for neurons, ~20ng for microglia, and ~10ng for astrocytes.cDNA was generated from up to 25 ng of total RNA using Nugenâs RNA-Seq method for low-input RNA samples, Ovation RNA-Seq System V2 (NuGEN). (Per manufacturers instructions, total RNA was neither depleted of rRNA nor polyA-selected.) 1 μg of sheared cDNA was taken into further processing, starting at end repair step, using Illuminaâs TruSeq RNA Sample Preparation Kit v2 (Illumina).The "SAMID" sample characteristic is a sample identifier internal to Genentech.The ID of this project in Genentech's ExpressionPlot database is PRJ0007717
提供机构：
Genentech, Inc.
创建时间：
2022-02-20
