RNA sequence reveal gene expression mechanism of sugar beet in response to alkaline stress

Here, we first systematically identified and characterized responsive lncRNAs and mRNAs expressed in leaves of sugar beet at a genome-wide scale, focusing on alkaline-responsive lncRNAs and mRNAs. Overall design: Five-week-old seedlings were treated with Hoagland solution, containing 75 mM mixed alkaline solution (Na2CO3:NaHCO3, 1:2) for 0 day (control, designated as C), 3 days (short-term treatment, designated as ST) and 7 days (long-term treatment, designated as LT). Fresh leaves from the same position of seedlings at different treatments were collected, and three biological replicates were sampled.