Elevated sleep quota in a stress-resilient Drosophila species

# Elevated sleep quota in a stress-resilient Drosophila species [https://doi.org/10.5061/dryad.w9ghx3fxw](https://doi.org/10.5061/dryad.w9ghx3fxw) Dataset contains underlying data for main and supplemental figures from manuscript. Included files: 1. Source data for Figure 1.xls 1. Baseline sleep time course data (Figure 1A), total day and night sleep (Figure 1B; values in minutes of sleep), P(wake) (Figure 1C), P(doze) (Figure 1D), and counts/waking minute (Figure 1E) for females from D. melanogaster and D. mojavensis stocks. Columns represent data from individual flies with stock label in Row 1. Rows represent 30-min time bins for Figure 1A, or day and night for Figures 1B-E. 2. Length of individual sleep bouts during the day (Figure 1F) and night (1G) from D. melanogaster and D. mojavensis stocks. 3. Daily sleep amount for D. melanogaster and D. mojavensis stocks using different time durations for minimum sleep bout length (Figure 1H; values in minutes of sleep). 4. Sleep time course for D. moj. moj. individuals across a 7-day experiment. Columns represent data from individual flies, rows show sleep in 30-min time bins (Figure 1I-J). 2. Source data for Figure 2.xls 1. Sleep time course (Figure 2A, left) and daily sleep total (Figure 2A, right; values in minutes of sleep) for three stocks of D. melanogaster 2. Sleep time course (Figure 2B, left) and daily sleep total (Figure 2B, right; values in minutes of sleep) for D. melanogaster and D. mojavensis stocks fed Banana-Cactus media 3. Sleep time course (Figure 2C, left) and daily sleep total (Figure 2C, right; values in minutes of sleep) for stocks of D. melanogaster and 4 species of desert-adapted flies (D. arizonae, D. buzzatii, D. mulleri & D. mojavensis) 4. Daily sleep totals for D. melanogaster and D. mojavensis housed at different constant temperatures from 25˚C to 31˚C (Figure 2D; values in minutes of sleep) 5. Absolute and relative sleep time courses for D. melanogaster and D. mojavensis flies exposed to a daily temperature ramp from 25˚C to 35˚C (Figures 2E & 2F) 3. Source data for Figure 3.xls 1. Sleep time course for D. moj. moj (Figure 3A) and D. moj baja (Figure 3B) for flies during one day of baseline, one day with overnight vortex sleep deprivation, and one recovery day (SD group) along with one group re-plotting the baseline sleep timecourse for visual comparison (Baseline replotted). Columns represent data from individual flies; rows are sleep in 30-min bins. 2. % of sleep recovered from D. melanogaster and D. mojavensis stocks after overnight vortex sleep deprivation (Figure 3C). 3. % of flies awakened (Figure 3D) and Latency to sleep (Figure 3E) for D. moj moj flies that were exposed to hourly blue LED stimulation. Columns show experimental group (Control, SD, SD+24h recovery), rows represent mean group responses for individual stimulus trials. 4. Sleep time course for D. moj. moj (Figure 3F) and D. moj. baja (Figure 3G) for flies during on one day of baseline, one day with overnight blue LED sleep deprivation, and one recovery day (Blue LED SD group) along with one group that did not receive blue LED stimulation (Control group). Columns represent data from individual flies; rows are sleep in 30-min bins. 5. % of sleep lost for D. moj moj and D. moj baja during overnight blue LED stimulation (Figure 3H). Columns show D. moj subspecies, rows show individual fly responses. 6. Daily sleep totals for experimental groups from Figure 3G and 3H (Figure 3I; values in minutes of sleep). Columns represent individual flies, rows show each day of experiment. 7. Sleep time course for D. moj moj (Figure 3J) and D. moj baja (Figure 3K) for flies housed in 12h:12h LD followed by 2-days in constant darkness. Daily sleep totals across the experiment in Figure 3L (values in minutes of sleep). 4. Source data for Figure 4.xls 1. Relative neurochemical abundance from D. melanogaster and D. mojavensis for 5HT (Figure 4A), OA (Figure 4B), DA (Figure 4C), and HA (Figure 4D). Values are normalized to mean from Canton-S D. melanogaster abundance for each assay. 2. Sleep timecourse (Figure 4K) for flies that received microinjections of octopamine or vehicle control. Total sleep and activity counts in 24h following injection in Figure L (values in minutes of sleep) & M. 5. Source data for Figure 6.xls 1. Survival timecourse for D. melanogaster (Cs) and D. mojavensis (moj and baja) during food deprivation (Figure 6A) or food and water deprivation (Figure 6B). 2. Daily sleep totals for D. moj baja (Figure 6C) and D. moj baja (Figure 6D) for flies that are either fed on standard fly media, food deprived, or food and water deprived. Values shown in minutes of sleep. 3. Survival times for D. moj baja flies that were either housed in 12h:12h LD or constant blue light during food deprivation (Figure 6E) 4. Scatterplot data for total sleep (values in minutes of sleep) vs survival time for individual D. moj baja flies during food deprivation (Figure 6F). 5. Survival times for D. moj baja flies that were either housed in 12h:12h LD or constant blue light during food and water deprivation (Figure 6G) 6. Scatterplot data for total sleep (values in minutes of sleep) vs survival time for individual D. moj baja flies during food and water deprivation (Figure 6H). 7. Survival times for D. moj moj flies that were either housed in 12h:12h LD or constant blue light during food deprivation (Figure 6I). 8. Daily sleep times for D. moj moj flies housed in 12h:12h LD or constant blue light during food deprivation (Figure 6J; values in minutes of sleep). 9. Survival times for responding vs non-responding D. moj moj flies housed in constant blue light during food deprivation (Figure 6K). 10. Scatterplot data for total sleep (values in minutes of sleep) vs survival time for individual D. moj moj flies during food and water deprivation (Figure 6L). 11. Survival times for D. moj moj flies that were either housed in 12h:12h LD or constant blue light during food and water deprivation (Figure 6M). 12. Daily sleep times for D. moj moj flies housed in 12h:12h LD or constant blue light during food and water deprivation (Figure 6N; values in minutes of sleep). 13. Survival times for responding vs non-responding D. moj moj flies housed in constant blue light during food and water deprivation (Figure 6O). 14. Scatterplot data for total sleep (values in minutes of sleep) vs survival time for individual D. moj moj flies during food and water deprivation (Figure 6P). 6. Source data for Figure S1.xls 1. Baseline sleep time course data (Figure S1A), total day and night sleep (Figure S1B; values in minutes of sleep), P(wake) (Figure S1C), P(doze) (Figure 1SD), and counts/waking minute (Figure S1E) for males from D. melanogaster and D. mojavensis stocks. Columns represent data from individual flies with stock label in Row 1. Rows represent 30-min time bins for Figure 1A, or day and night for Figures 1B-E. 7. Source data for Figure S3.xls 1. Sleep time course for D. melanogaster (Cs) and D. mojavensis females while flies were housed at 25˚C for one day followed by three days at 27˚C (Figure S3A), 29˚C (Figure S3B), or 31˚C (Figure S3C). Columns represent data from individual flies, rows show sleep time/30-min bin. 8. Source data for Figure S4.xls 1. P(wake) values for individual D. moj moj (Figure S4A) or D. moj baja (Figure S4B) during the day and night on a baseline day and recovery day after 12h overnight vortex sleep deprivation. 2. Activity counts/waking minute values for individual D. moj moj (Figure S4C) or D. moj baja (Figure S4D) during the day and night on a baseline day and recovery day after 12h overnight vortex sleep deprivation. 3. Sleep timecourse for D. moj moj (Figure S4E) or D. moj baja (Figure S4F) for flies during two days of recovery after 12h overnight vortex sleep deprivation (Recovery days (after overnight vortex SD) group) or replotting of sleep time from baseline days for visual comparison (Baseline replotted group). 9. Source data for Figure S6.xls 1. Survival times for fed D. moj moj and D. moj baja flies housed either in 12h:12h LD or constant blue light (Figure S6A). 2. Daily sleep time (values in minutes of sleep) for D. moj baja flies housed in either 12h:12h LD or constant blue light during food deprivation (Figure S6B) or food and water deprivation (Figure S6C). 3. Scatter plot data for D. moj baja flies during food deprivation: Daily activity counts vs Survival time (hrs) (Figure S6D); Activity counts/waking minute vs Survival time (hrs) (Figure S6E); Daily sleep (values in mins of sleep) vs Daily activity counts (Figure S6F). 4. Scatter plot data for D. moj baja flies during food and water deprivation: Daily activity counts vs Survival time (hrs) (Figure S6G); Activity counts/waking minute vs Survival time (hrs) (Figure S6H); Daily sleep (values in mins of sleep) vs Daily activity counts (Figure S6I). 5. Scatter plot data for D. moj moj flies during food deprivation: Daily activity counts vs Survival time (hrs) (Figure S6J); Activity counts/waking minute vs Survival time (hrs) (Figure S6K); Daily sleep (values in mins of sleep) vs Daily activity counts (Figure S6L). 6. Scatter plot data for D. moj moj flies during food and water deprivation: Daily activity counts vs Survival time (hrs) (Figure S6M); Activity counts/waking minute vs Survival time (hrs) (Figure S6N); Daily sleep (values in mins of sleep) vs Daily activity counts (Figure S6O).