Sample collection from in-patients can be challenging and potentially impacted by collection method variability. We used 16S rRNA-based sequencing to compare the microbiota profiles of variably stored
Epidemiologic studies use various biosample collection methods to study associations between human oral microbiota and health outcomes. However, the agreement between the different methods is unclear.
In this study, we examine several commonly used protocols for extracting bacterial DNA from ants to assess the biases inherent in each method. Bacterial community composition recovered using Illumina
Herein, we evaluate different methodological set-ups to detect and quantify archaea signatures in human samples by amplicon sequencing and quantitative PCR. We used five different sample types to eval
we evaluated the bias of nested PCR in estimating microbial diversity and community structure by comparing it with standard PCR. We investigated the influence of microbial diversity (relatively low vs
